The United States described the release of the prisoners on humanitarian grounds as the result of months of pressure and said it did not intend to let up on Ortega.

"Thank you to everyone for my freedom. Long live a free Nicaragua!" Francisco Arteaga told reporters as he raised his fist from a bus window on landing in Guatemala City.

Arteaga said he had been jailed for his social media postings about Ortega's actions against the Catholic Church, which the leftist leader has increasingly treated as a threat.

The White House said that the prisoners -- all Nicaraguan citizens -- included members of faith organizations, students and others viewed by Ortega and his team as a "threat to their authoritarian rule."

They will have the opportunity to seek to move to the United States, which plans to support them during their stay in Guatemala, whose reformist President Bernardo Arevalo has worked closely with Washington on the hot-button political issue of migration.

"We urge the immediate and unconditional release of all political prisoners and prisoners of conscience in Nicaragua," Secretary of State Antony Blinken said in a statement.

"The Nicaraguan people want and deserve a restored democracy where all can exercise their human rights and fundamental freedoms, free from fear of persecution or reprisal."

The latest move follows the mass release of more than 200 prisoners in February 2023 who were flown to the United States.

The former prisoners have largely welcomed being able to leave, although some human rights activists view Ortega's move cynically, seeing him as trying to get rid of perceived opponents while at least attempting to curry favor with Washington.

Eric Jacobstein, a senior State Department official involved in the latest release, said that "the Nicaraguan regime got nothing from this."

"I do want to emphasize that this does not signal a change in our policy toward this repressive regime," Jacobstein told reporters.

Ortega and his allies "continue to suppress democracy, fail to respect the human rights of Nicaraguans and deepen Nicaragua's collaboration with authoritarian governments," he said, calling Nicaragua's growing relations with Russia and China "extremely concerning."